Microalgal heterotrophic bioreactors are a potential technological development that can convert organic matter, nitrogen and phosphorus of wastewaters into a biomass suitable for energy production. However, the commercial production of microalgal biodiesel was halted due to its high cost. Biomass yields in heterotrophic and mixotrophic conditions 15. Fermentors are mainly used to produce heterotrophic microalgae using. Comparison of autotrophic and mixotrophic cultivation of. Biodiesel production from chlorella protothecoides oil by. Microalgal feedstocks have been considered a suitable alternative to traditional oil.
Also, algae fuels are an alternative to commonly known biofuel sources, such as corn and sugarcane. Biodiesel production from heterotrophic microalgae. Kinetic studies and thermodynamics of oil extraction and. Heterotrophic oil production from microalgae greatly exceeds that of vegetable oil crops. Large amount of microalgal oil was efficiently extracted from these heterotrophic cells by using nhexane. Biomass and lipid production of heterotrophic microalgae. Biodiesel fuel production from algae as renewable energy. Global increases in atmospheric co2 and climate change are drawing considerable attention to identify sources of energy with lower environmental impact than those currently in use. Miao and wu studied biodiesel production from heterotrophic cultivated microalgae oil from c. In this work, microgreen algae of chlorella vulgaris tistr 8580 was cultivated under heterotrophic conditions of no light intensity in the dark, carbon 10 g l. In this paper, the different sources of energy such as oil, conventional and unconventional. Microalgal biotechnology appears to possess high potential for biodiesel production because a significant increase in lipid content of microalgae is now possible through heterotrophic cultivation. Through substrate feeding and fermentation process controls, the cell density of c.
Although numerous reports are available on the production of biodiesel from vegetable oils of terraneous oil plants, such as soybean, sun. Heterotrophic growth of chlorella protothecoides resulted. Here, we presented a pilot study on the industrial production of algal biodiesel. Algal companies specialized in biodiesel production the following is a list of companies developing processes for the production of biodiesel from algae oil. Producing biofuel from microalgae has gained renewed interest recently.
Pdf biodiesel production from heterotrophic microalgal oil. Heterotrophic growth of chlorella protothecoides resulted in the accumulation of high lipid. Screening, growth medium optimisation and heterotrophic. Heterotrophic cultivation of microalgae for production of biodiesel volume. The aim of this work was to evaluate the performance of microalgal heterotrophic bioreactors in the secondary treatment of cattleslaughterhouse. Biodiesel comparable to conventional diesel was obtained from heterotrophic microalgal oil by acidic transesterification. Biodiesel optimum conversion yield of 63% was obtained with 56. We began with the heterotrophic cultivation of auxenochlorella protothecoides in a 60m3 fermentor that produced biomass at 3. Fermentors are mainly used to produce heterotrophic microalgae using an organic. Changes in the right angle light scatter rals and forward angle light scatter fals were detected during the microalgal growth, which were attributed to the different microalgal cell cycle stages. An acid catalyst hydrochloric acid was used in this research because the alkali catalyst was not suitable for the transesterification of microalgal oil, most likely because of the high acid value of microalgal oil.
Schizochytrium limacinum is a heterotrophic microalga that is capable of producing high levels of biomass and total fatty acid. The lipidtomethanol molar ratio sratio is an important parameter for lipid extraction, but its effects on product yield were found insignificant between levels of sratio due to the higher ratios tested in this study. Chlorella protothecoides, biodiesel, symbiosis, autotrophic, heterotrophic 1. Potential assessment of microalgal oils for biodiesel. It is possible to produce adequate microalgal biofuels to satisfy the fast growing energy demand within the restraints of land and water resources. Biodiesel lipids fatty acids production abstract biodiesel has received much attention in recent years. Modeling the production of microalgal biodiesel mark henson washington university in st. These include methane produced by anaerobic digestion of the algal biomass6 biodiesel derived from microalgal oil 11,12, and. Production of methyl esters, or biodiesel, from microalgal oil has been demonstrated belarbi et al. In this paper, the different sources of energy such as oil, conventional and. Heterotrophic cultivation of microalgae for lipids production.
Biodiesel produced from microalgae will not compromise the production of food and other products derived from crops. Heterotrophic cultivation of microalgae for production of. B1 in which three fatty acid molecules are esterified with a molecule of glycerol. Biodiesel production by microalgal biotechnology rvo. Frontiers industrial fermentation of auxenochlorella. In situ biodiesel production from fastgrowing and high oil. However, the microalgal productivity can easily attain 1. In situ transesterification of microalgal oil to produce algal biodiesel 1 executive summary microalgae are promising candidate biomass resources as potential feedstocks for renewable energy production. At present, algal oil represents less than 2% of human epadha. Progress and challenges in microalgal biodiesel production. When made from seaweed macroalgae it can be known as seaweed fuel or seaweed oil several companies and government agencies are funding efforts to reduce capital. The objective of this work is to produce heterotrophic microalgal lipid in flaskbatch fermentation. Multiparameter flow cytometry as a tool to monitor. In this study, biodiesel production from microalgal oil by microwaveassisted transesterification was carried out to investigate its efficiency.
Biodiesel production from microalgae lipids can, in the future, occupy a prominent place in energy generation because it represents a sustainable alternative to petroleumbased fuels. This report assesses the economics of microalgae biofuels production. Microalgae biodiesel pdf institute of technology and engineering, massey university, private bag 11. Crude microalgal oil was employed in this study because the oil purification processes impose extra expenses and increase the final cost of the product. This dissertation is brought to you for free and open access by washington university open scholarship. However, commercial application of biodiesel production from c.
Biodiesel production from heterotrophic microalgal oil sciencedirect. Introduction more oil for biodiesel production than traditional crops on an nowadays, about 80% of global energy demand is. Oct 01, 2008 in this work, microgreen algae of chlorella vulgaris tistr 8580 was cultivated under heterotrophic conditions of no light intensity in the dark, carbon 10 g l. Biofuels from microalgae li 2008 biotechnology progress. Biodiesel comparable to conventional diesel was obtained from. Comparison of autotrophic and heterotrophic cultivations of. Further efforts in microalgae screening for biodiesel production are needed to discover a native microalgae that will be feasible for biodiesel production in terms of biomass productivity and oil. Jun 30, 2016 currently, biodiesel production from microalgal biomass has drawn an immense importance with the potential to exclude highquality agricultural land use and food safekeeping issues. Biofuel production by oleaginous microalgae is a promising alternative to.
This article presents a study on screening of microalgal strains from the peking university algae collection and heterotrophic cultivation for biodiesel production of a selected microalgal strain. Delft university of technology biodiesel production from. Investigations on microalgal oil production from arthrospira. Furthermore, microalgal biodiesel production is restricted mostly due to the high cost of the fermentation substrate, while the cost of glucose accounted for 80% of the total medium cost li et al. These include methane produced by anaerobic digestion of the algal biomass6 biodiesel derived from microalgal oil11,12, and. Although numerous reports are available on the production of biodiesel from vegetable oils of terraneous oilplants, such as soybean, sun. The quality of the oil produced from the cells of heterotrophic growth is also superior compared the oil from photoautotrophic growth. Pdf biodiesel production from heterotrophic microalgal. Microalgal biofuel production is potentially sustainable. They have been investigated for the production of different biofuels including biodiesel, bio. Microalgae has much more oil than macroalgae and it is much faster and easier to grow10. Biodiesel production potential of wastewater microalgae. The characteristics of acidic transesterification of microalgal oil and the properties of the biodiesel were investigated.
Production of single cell oil for biodiesel from microalgae. Pdf production of biodiesel from microalgae researchgate. Enzymatic production of biodiesel from microalgal oil. Heterotrophic cultivation of microalgae for production of biodiesel.
Pdf multiparameter flow cytometry as a tool to monitor. The objective of this work is to explore the potential of producing biodiesel fuel from this alga using different biodiesel preparation methods, including oil extraction followed by. Heterotrophic culture of microalgae using biodiesel derived glycerol a dissertation presented to the graduate school of clemson university in partial fulfillment of the requirements for the degree doctor of philosophy biosystems engineering. A similar fame profile was also found for both biodiesel samples, comprising c16. Moreover, microalgae can grow in seawater or wastewater and microalgal oil can exceed 5060% dry cell weight as compared with some best agricultural oil crops. Biodiesel production by microalgal biotechnology mafiadoc. Ratanaporn leesing, supaporn kookkhunthod, ngarmnit nontaso abstract.
Pufa content in the microalgal oils was negligible under the investigated conditions, which confirms the potential of biodiesel production from spirulina. High cell density, heterotrophic microalgae, axenic. Microalgae have high potentials in biodiesel production compared to other oil crops. Production of biodiesel from microalgae through mafiadoc. Microalgae have high biomass productivity, high lipid content, low cultivation cost, and no competition with agricultural land. First, the cultivation of microalgae dose not need much land as compared to that of terraneous plants. The effect of ethyl acetate on the stability of commercial immobilized lipase, novozym 435, was also investigated. Based on the quality and quantity standard of biodiesel, the optimized temperature and the reaction time for in situ biodiesel production from microalgal biomass were 90c and 2 hours. Comparison of autotrophic and heterotrophic cultivations. Among 89 strains, only five were capable of growing under heterotrophic conditions in liquid cultures and chlorella sp. May 21, 2014 this article presents a study on screening of microalgal strains from the peking university algae collection and heterotrophic cultivation for biodiesel production of a selected microalgal strain.
Microalgae can provide several different types of renewable biofuels. Pkuac 102 was found the best for the production of heterotrophic algal biodiesel. Converting the algal oil to biofuels biodiesel, green diesel, jet fuel may be the lesser challenge. Nano 3 sources to induce oil accumulation in its cell and compared to the controlled or natural condition of autotrophic cultivation supplemented with light intensity of. Heterotrophic culture of microalgae using biodiesel. Transesterification reactions were performed by using chlorella protothecoides oil as feedstock, methanol, and potassium hydroxide as the catalyst.
In making biodiesel, triglycerides are reacted with. Multiparameter flow cytometry as a tool to monitor heterotrophic microalgal batch fermentations for oil production towards biodiesel. Algae fuel, algal biofuel, or algal oil is an alternative to liquid fossil fuels that uses algae as its source of energyrich oils. Treatment of cattleslaughterhouse wastewater and the. Apr 16, 2019 nutrient removal and microalgal biomass production from different anaerobic digestion effluents with chlorella species. Biodiesel production by microalgal biotechnology request pdf. Jul 14, 2009 multiparameter flow cytometry was used to monitor cell intrinsic light scatter, viability, and lipid content of chlorella protothecoides cells grown in shake flasks.
In the present study, an integrated method for biodiesel production from heterotrophic microalgal oil by acidic transesterification was developed. Nutrient removal and microalgal biomass production from different anaerobic digestion effluents with chlorella species. Figure 5 effects of temperature and reaction time on the biodiesel yield by in situ transesterification of c. Pkuac 102 was found the best for the production of heterotrophic algal. The quality of the oil produced from the cells of heterotrophic growth is also superior compared the oil. When made from seaweed macroalgae it can be known as seaweed fuel or seaweed oil. Production of biodiesel from microalgae intechopen.
This revision focuses in the biotechnological potential and viability of biodiesel production from microalgae. Treatment of cattleslaughterhouse wastewater and the reuse. High cell density cultivation of microalgae via heterotrophic growth mechanism could effectively address the issues of low productivity and operational constraints presently affecting the solar driven biodiesel production. Multiparameter flow cytometry was used to monitor cell intrinsic light scatter, viability, and lipid content of chlorella protothecoides cells grown in shake flasks. Biodiesel production from heterotrophic microalgal oil. Other limitations include the low concentrations of free co 2 that are required for peak algal growth and that algal grazers are a significant, but. An integrated approach of biodiesel production from heterotrophic chlorella protothecoides focused on scaling up fermentation in bioreactors was reported in this study. Department of bioprocess technology, faculty of biotechnology and biomolecular sciences, universiti putra malaysia, 43400 upm serdang, selangor, malaysia. Introduction more oil for biodiesel production than traditional crops on an. Microalgal lipid production by microalgae chlorella sp.
809 32 1502 1509 205 556 657 1406 272 1322 120 646 1096 681 1178 801 758 674 800 1520 572 468 1481 206 1338 3 376 852 1512 402 1056 1413 438 68 1062 140 1230 636 1462